Biology- Biotechnology Principles and Processes

1.

In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be

both transformants and non-transformants cannot survive. both transformants and non-transformants can survive. transformants only and not the non-transformants can survive. transformants cannot survive, but non-transformants can not.

2.

'Restriction' in Restriction enzyme refers to

cleaving ofphosphodiester bond in DNA by the enzyme cutting of DNA at specific position only prevention of the multiplication of bacteriophage in bacteria all of the above

3.

The role of DNA ligase in the construction of a recombinant DNA molecule is

formation of phosphodiester bond between two DNA fragments formation of hydrogen bonds between sticky ends of DNA fragments ligation of all purine and pyrimidine bases none of the above

4.

Plasmids in bacterial cells are

extra-chromosomal DNA, which cannot replicate extra-chromosomal DNA, which can . self-replicate extra DNA associated with the genome extra DNA, associated with the genome, but cannot replicate.

5.

The DNA polymerase enzyme used in PCR is obtained from

Thermus aquaticus Escherichia coli Agrobacterium tumefaciens Salmonella typhimurium.

6.

While isolating DNA from bacteria, which of the following enzymes is not used?

Lysozyme Ribonuclease Deoxyribonuclease Protease

7.

Significance of 'heat shock' method in bacterial transformation is to facilitate

binding of DNA to the cell wall uptake of DNA through membrane transport proteins uptake of DNA through transient pores in the bacterial cell wall expression of antibiotic resistance gene

8.

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

Denaturation of template DNA Annealing of primers to template DNA Extension of primer end on the template DNA All of the above

9.

Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

DNA can be seen in visible light DNA can be seen without staining in visible light Ethidium bromide stained DNA can be seen in visible light Ethidium bromide stained DNA can be seen under exposure to UV light

10.

Biolistics (gene gun) is suitable for

introducing rDNA into plant cells introducing rDNA into animal cells disarming the pathogen vectors DNA fingerprinting.

Biology- Biotechnology Principles and Processes

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